ACELL June 45/6
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چکیده
Tian, Jean Q., and Andrea Quaroni. Involvement of p21(WAF1/Cip1) and p27(Kip1) in intestinal epithelial cell differentiation. Am. J. Physiol. 276 (Cell Physiol. 45): C1245– C1258, 1999.—Using the conditionally immortalized human cell line tsFHI, we have investigated the role of cyclindependent kinase inhibitors (CKIs) in intestinal epithelial cell differentiation. Expression of cyclins, cyclin-dependent kinases (Cdk), and CKIs was examined under conditions promoting growth, growth arrest, or expression of differentiated traits. Formation of complexes among cell cycle regulatory proteins and their kinase activities were also investigated. The tsFHI cells express three CKIs: p16, p21, and p27. With differentiation, p21 and p27 were strongly induced, but with different kinetics: the p21 increase was rapid but transient and the p27 increase was delayed but sustained. Our results suggest that the function of p16 is primarily to inhibit cyclin D-associated kinases, making tsFHI cells dependent on cyclin E-Cdk2 for pRb phosphorylation and G1/S progression. Furthermore, they indicate that p21 is the main CKI involved in irreversible growth arrest during the early stages of cell differentiation in association with D-type cyclins, cyclin E, and Cdk2, whereas p27 may induce or stabilize expression of differentiated traits acting independently of cyclin-Cdk function.
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ACELL June 45/6
J. D. PAL,1 V. M. BERTHOUD,2 E. C. BEYER,2 D. MACKAY,3 A. SHIELS,3 AND L. EBIHARA1 1Department of Physiology and Biophysics, Finch University of Health Sciences/The Chicago Medical School, North Chicago 60064; 2Department of Pediatrics, University of Chicago, Chicago, Illinois 60637; and 3Department of Molecular Genetics, Institute of Ophthalmology, Washington University School of Medicine, St....
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Villalobos, Alice R., Judith T. Parmelee, and J. Larry Renfro. Choline uptake across the ventricular membrane of neonate rat choroid plexus. Am. J. Physiol. 276 (Cell Physiol. 45): C1288–C1296, 1999.—The uptake of [3H]choline from the cerebrospinal fluid (CSF) side of the rat neonatal choroid plexus was characterized in primary cultures of the choroidal epithelium grown on solid supports. Cell-...
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MARK O. BEVENSEE,1 ESTHER BASHI,1 WOLF-RÜDIGER SCHLUE,2 GREGORY BOYARSKY,3 AND WALTER F. BORON1 1Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, Connecticut 06520; 2Institut für Neurobiologie, Heinrich-Heine-Universität Düsseldorf, 40225 Düsseldorf, Germany; and 3Department of Physiology and Biophysics, University of Texas Medical Branch, Galvesto...
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Berry, Susan A., Pearl L. Bergad, Allison M. Stolz, Howard C. Towle, and Sarah Jane Schwarzenberg. Regulation of Spi 2.1 and 2.2 gene expression after turpentine inflammation: discordant responses to IL-6. Am. J. Physiol. 276 (Cell Physiol. 45): C1374–C1382, 1999.—The rat serine protease inhibitor (Spi) 2 gene family includes both positive (Spi 2.2) and negative (Spi 2.1) acute phase reactants,...
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AKI YAMADA,1 SUSUMU OHYA,2 MASARU HIRANO,2 MINORU WATANABE,2 MICHAEL P. WALSH,3 AND YUJI IMAIZUMI1 1Department of Pharmacology and Therapeutics and 2Department of Chemical Pharmacology, Faculty of Pharmaceutical Sciences, Nagoya City University, Nagoya 467-8603, Japan; and 3Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Calgary, Calgary, Alberta T2N 4N1, Ca...
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